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Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

Frontiers of Medicine 2023, Volume 17, Issue 3,   Pages 493-502 doi: 10.1007/s11684-022-0946-x

Abstract: Anaplastic lymphoma kinase (<i>ALKi>) is the most common fusion gene involved in non-small cell lungIn this work, we investigated whether the variant allele frequencies (VAFs) of <i>ALKi> fusions canALKi> positive.ALKi> subclonality with crizotinib efficacy was assessed.Results suggest that the <i>ALKi> VAF determined by hybrid capture-based NGS is probably unreliable

Keywords： <i>ALKi> fusion     next-generation sequencing     fluorescence <i>in situi> hybridization     immunohistochemistry    

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Niche Differentiation of Phenol-Degrading Microorganisms in UASB Granular Sludge as Revealed by Fluorescence<i>in situi> Hybridization Article

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

Engineering 2022, Volume 9, Issue 2,   Pages 61-66 doi: 10.1016/j.eng.2021.05.012

Abstract: harvested from an anaerobic sludge blanket reactor treating phenolic wastewater was investigated using fluorescencein situ hybridization (FISH) and clone library construction.

Keywords： Cryptanaerobacter     Fluorescence     in situ     hybridization     Anaerobic phenol degradation     Syntrophorhabdaceae    

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hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 1,   Pages 92-102 doi: 10.1007/s11705-021-2063-9

Abstract: Herein, we constructed an <i>in situi> hydrophobic environment-triggering reactive fluorescence probeThe fluorophore was 2-styrene-1<i>Hi>-benzo[<i>di>]imidazole, and malononitrile was introduced asThus, MBI-CN can be hydrolyzed to generate MBI-CHO in an <i>in situi> hydrophobic environment withMeanwhile, MBI-CHO immediately emitted a significative fluorescence signal changes at 437 and 553 nm

Keywords： hydrolysis reaction     mitochondrial DNA damage     <i>in situi> hydrophobic environment trigger     fluorescence    

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Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 1,   Pages 48-58 doi: 10.15302/J-FASE-2016114

Abstract: Wheat stripe rust caused by f. sp. is one of the most devastating diseases of wheat worldwide and resistant cultivars are vital for its management. Therefore, investigating the heterozygosity of the pathogen is important because of rapid virulence changes in isolates heterozygous for avirulence/virulence. An isolate of f. sp. was selfed on to determine the heterozygosity for avirulence/virulence loci. One hundred and twenty progeny isolates obtained from this selfing were phenotyped using 25 lines of wheat containing genes and genotyped with 96 simple sequencing repeat markers, with 51 pathotypes and 55 multi-locus genotypes being identified. All of these were avirulent on lines with , , , and and virulent on lines with , and , indicating that the parental isolate was homozygously avirulent or homozygously virulent for these loci. Segregation was found for wheat lines with , , , , , , , , , , , , , , , and . The 17 cultivars to which the was identified as heterozygous with respect to virulence/avirulence should not be given priority in breeding programs to obtain new resistant cultivars.

Keywords： <i>Puccinia striiformisi> f. sp. <i>triticii>     selfing     heterozygosity     virulence inheritance    

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Development of an H reduction and moderate oxidation method for 3,5-dimethylpyridine hydrogenation in trickle bed reactor

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 12,   Pages 1807-1817 doi: 10.1007/s11705-022-2243-2

Abstract: i> reduced Ru/C-n catalyst was higher than that of the <i>in-situi> reduced Ru/C-y catalyst.Therefore, an <i>in-situi> H2 reduction and moderate oxidation method was developed to increaseof the <i>in-situi> H2 reduction and moderate oxidized Ru/C-i2 catalyst, the interactionThe developed <i>in-situi> H2 reduction and moderate oxidation method eliminated the stepof the non-<i>in-situi> reduction of catalyst outside the trickle bed reactor.

Keywords： Ru/C catalyst     <i>in-situi> H2 reduction and moderate oxidation     <i>in-situi> reduction     non-<i>in-situi> reduction     hydrogenation of 3     5-dimethylpyridine    

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A review on

Shaolong WAN,Yong WANG

Frontiers of Chemical Science and Engineering 2014, Volume 8, Issue 3,   Pages 280-294 doi: 10.1007/s11705-014-1436-8

Abstract: Catalytic fast pyrolysis (CFP) is deemed as the most promising way to convert biomass to transportation fuels or value added chemicals. Most works in literature so far have focused on the CFP where the catalysts are packed or co-fed with the feedstock in the pyrolysis reactor. However, the CFP with catalysts separated from the pyrolyzer has attracted more and more attentions due to its unique advantages of individually optimizing the pyrolysis conditions and catalyst performances. This review compares the differences between the and CFP operation, and summarizes the development and progress of CFP applications, including the rationale and performances of different catalysts, and the choices of suitable reactor systems. Due to the complex composition of bio-oil, no single approach was believed to be able to solve the problems completely among all those existing technologies. With the increased understanding of catalyst performances and reaction process, the recent trend toward an integration of biomass or bio-oil fractionation with subsequent thermo/bio-chemical conversion routes is also discussed.

Keywords： catalytic fast pyrolysis     <i>ex situi>     catalysts    

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Development of lunar regolith-based composite for - 3D printing via high-pressure extrusion system

Frontiers of Mechanical Engineering 2023, Volume 18, Issue 2, doi: 10.1007/s11465-022-0745-8

Abstract: To fully utilize the <i>in-situi> resources on the moon to facilitate the establishment of a lunarini>-<i>situi> construction.However, the <i>ini>-<i>situi> 3D printing using raw lunar regolith consumes extremely high energyini>-<i>situi> resources of lunar regolith.ini>-<i>situi> manufacturing.

Keywords： <i>ini>-<i>situi> resource utilization     melt extrusion molding     lunar regolith-based composites    

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Development of an <i>Ini>-<i>Situi> Laser Machining System Using a Three-Dimensional Galvanometer Article

Xiao Li, Bin Liu, Xuesong Mei, Wenjun Wang, Xiaodong Wang, Xun Li

Engineering 2020, Volume 6, Issue 1,   Pages 68-76 doi: 10.1016/j.eng.2019.07.024

Abstract: font-family:Arial,Helvetica,sans-serif;">In this study, a three-dimensional (3D) <i>ini>-<i>situi> laser machining system integrating laser measurement and machining was built using<i>Ini>-<i>situi> machining software was developed to realize time-saving and labor-saving 3D laserThe feasibility and practicability of this <i>ini>-<i>situi> laser machining system were verifiedThe <i>ini>-<i>situi> laser machining system realizes a simple operation process by integrating measurement

Keywords： <i>Ini>-<i>situi> laser machining     Three-dimensional galvanometer scanner     Line structured light    

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SWIR Fluorescence Imaging <i>In Vivoi> Monitoring and Evaluating Implanted M2 Macrophages in Skeletal Article

Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen

Engineering doi: 10.1016/j.eng.2023.05.010

Abstract: Here, we present a short-wave infrared (SWIR) fluorescence imaging technique to obtain more in vivoSWIR fluorescence imaging was employed to track implanted M2Mø in the injured skeletal muscleThen, SWIR fluorescence imaging of blood vessels showed that M2Mø implantation could improve the

Keywords： <i>In vivoi>     Short-wave infrared     Skeletal muscle     Macrophage     Regeneration    

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><i>In situi> growth of phosphorized ZIF-67-derived amorphous CoP/Cu2O@CF electrocatalyst

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 10,   Pages 1430-1439 doi: 10.1007/s11705-023-2320-1

Abstract: Herein, we report an <i>in situi> phosphorization of zeolitic imidazole frameworks (ZIF-67) to generateFirst-principle calculations illustrated that benefit from the construction of CoP/ZIF-67 heterojunction, the <i>di>-band center of CoP downshifted after bonding with ZIF-67 and the Gibbs free energy (Δ<i>Gi>

Keywords： CoP/Cu2O@CF     electrocatalyst     phosphorization     HER     DFT    

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Development of lunar regolith composite and structure via laser-assisted sintering

Frontiers of Mechanical Engineering doi: 10.1007/s11465-021-0662-2

Abstract: Aiming at the exploration and resource utilization activities on the Moon, <i>in situi> resource utilizationand <i>in situi> manufacturing are proposed to minimize the dependence on the ground transportation

Keywords： <i>in situi> manufacturing     laser-assisted powder fusion process     mechanical properties     topological    

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CeO doping boosted low-temperature NH-SCR activity of FeTiO catalyst: A microstructure analysis and reaction mechanistic study

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 5, doi: 10.1007/s11783-022-1539-2

Abstract: sub>2 doping significantly improved low-temperature NH3-SCR activity on FeTiO<i>xi>.

Keywords： NH3-SCR     CeO2 doping     Low-temperature NO<i>xi> removal     Improved redoxproperty     <i>In situi> XAFS analysis    

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density measurement for plastic injection molding via ultrasonic technology

Frontiers of Mechanical Engineering 2022, Volume 17, Issue 4, doi: 10.1007/s11465-022-0714-2

Abstract: Therefore, <i>in-situi> density measurement is of great interest and has significant application valueThis study is the first to propose an <i>in-situi> density measurement method using ultrasonic technologyThe proposed method has the advantages of <i>in-situi> measurement, non-destructive, high accuracy,

Keywords： ultrasonic measurement     melt density     <i>in-situi> measurement     injection molding    

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Development of Perennial Wheat Through Hybridization Between Wheat and Wheatgrasses: A Review Review

Lei Cui,Yongkang Ren,Timothy D. Murray,Wenze Yan,Qing Guo,Yuqi Niu,Yu Sun,Hongjie Li

Engineering 2018, Volume 4, Issue 4,   Pages 507-513 doi: 10.1016/j.eng.2018.07.003

Abstract:

Wheatgrasses (Thinopyrum spp.), which are relatives of wheat (Triticum aestivum L.), have a perennial growth habit and offer resistance to a diversity of biotic and abiotic stresses, making them useful in wheat improvement. Many of these desirable traits from Thinopyrum spp. have been used to develop wheat cultivars by introgression breeding. The perennial growth habit of wheatgrasses inherits as a complex quantitative trait that is controlled by many unknown genes. Previous studies have indicated that Thinopyrum spp. are able to hybridize with wheat and produce viable/stable amphiploids or partial amphiploids. Meanwhile, efforts have been made to develop perennial wheat by domestication of Thinopyrum spp. The most promising perennial wheat–Thinopyrum lines can be used as grain and/or forage crops, which combine the desirable traits of both parents. The wheat–Thinopyrum lines can adapt to diverse agricultural systems. This paper summarizes the development of perennial wheat based on Thinopyrum, and the genetic aspects, breeding methods, and perspectives of wheat–Thinopyrum hybrids.

Keywords： <i>Thinopyrumi>     Wheatgrass     Perennial     <i>Triticum aestivumi>    

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Hormesis-like growth and photosynthetic physiology of marine diatom Bohlin exposed to polystyrene microplastics

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 1,   Pages 2-2 doi: 10.1007/s11783-021-1436-0

Abstract:

• Polystyrene microplastic caused hormesis-like effects in <i>Phaeodactylumtricornutumi>.

Keywords： Microplastics     Marine microalgae     <i>Phaeodactylum tricornutumi> Bohlin     Growth     Photosynthetic pigments     Chlorophyll fluorescence parameters    

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Title Author Date Type Operation

Potential unreliability of ALK variant allele frequency in the efficacy prediction of targeted therapy in NSCLC

Journal Article

Niche Differentiation of Phenol-Degrading Microorganisms in UASB Granular Sludge as Revealed by Fluorescence<i>in situi> Hybridization

Kengo Kubota, Kei Igarashi, Masayoshi Yamada, Yasuyuki Takemura, Yu-You Li, Hideki Harada

Journal Article

hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA

Journal Article

Determination of heterozygosity for avirulence/virulence loci through sexual hybridization of

Yuan TIAN,Gangming ZHAN,Xia LU,Jie ZHAO,Lili HUANG,Zhensheng KANG

Journal Article

Development of an H reduction and moderate oxidation method for 3,5-dimethylpyridine hydrogenation in trickle bed reactor

Journal Article

A review on

Shaolong WAN,Yong WANG

Journal Article

Development of lunar regolith-based composite for - 3D printing via high-pressure extrusion system

Journal Article

Development of an <i>Ini>-<i>Situi> Laser Machining System Using a Three-Dimensional Galvanometer

Xiao Li, Bin Liu, Xuesong Mei, Wenjun Wang, Xiaodong Wang, Xun Li

Journal Article

SWIR Fluorescence Imaging <i>In Vivoi> Monitoring and Evaluating Implanted M2 Macrophages in Skeletal

Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen

Journal Article

><i>In situi> growth of phosphorized ZIF-67-derived amorphous CoP/Cu2O@CF electrocatalyst

Journal Article

Development of lunar regolith composite and structure via laser-assisted sintering

Journal Article

CeO doping boosted low-temperature NH-SCR activity of FeTiO catalyst: A microstructure analysis and reaction mechanistic study

Journal Article

density measurement for plastic injection molding via ultrasonic technology

Journal Article

Development of Perennial Wheat Through Hybridization Between Wheat and Wheatgrasses: A Review

Lei Cui,Yongkang Ren,Timothy D. Murray,Wenze Yan,Qing Guo,Yuqi Niu,Yu Sun,Hongjie Li

Journal Article

Hormesis-like growth and photosynthetic physiology of marine diatom Bohlin exposed to polystyrene microplastics

Journal Article